High performance, ion‐exchange chromatography of amino‐acids in biological fluids using Chromakon 500‐ performance of the apparatus

Luc Cynober,Frédéric Ziegler,Colette Coudray-Lucas,Jacqueline Giboudeau

doi:10.1155/s146392468500044x

Abstract

High performance, ion‐exchange chromatography of amino‐acids in biological fluids using Chromakon 500‐ performance of the apparatus

Highlights

The separation and quantitative measurement of aminoacids by ion-exchange chromatography has been in use for many years ]
All the reagents necessary for the ion-exchange chromatography were supplied by Merck (Darmstadt, FR Germany)
The stability of retention times was studied by 10 consecutive injections of the calibration solution

Summary

Introduction

The separation and quantitative measurement of aminoacids by ion-exchange chromatography has been in use for many years ]. The 40 amino-acid standard solution was supplied by Pierce and was supplemented with glutamine (final concentration 250 mol/1) from Calbioehem (San Diego, California, USA). Amino-acids were separated on the Chromakon 500 equipped with an automatic injection loop, a 15 cm column packed with Kontron AS 70 resin (diameter 7 ) and with five citrate buffers (see table 1).

Results

Conclusion