High Performance Genetically Encoded Biosensors of Cell Metabolism

Abstract

The Campbell research group is focused on the use of protein engineering for the development of high performance fluorescent protein-based biosensors for imaging of cell signalling and metabolism. Protein engineering, using a combination of rational protein design and directed protein evolution, is the most effective and versatile approach for generating new genetically encoded fluorescent biosensors. Indeed, by exploiting iterative cycles of high-throughput fluorescence image-based screening of bacterial colonies, and lower throughput testing of promising variants in mammalian cells, the Campbell group has developed a growing selection of fluorescent protein-based biosensors with improved properties. In this seminar I will present some of our most recent efforts to engineer an improved generation of biosensors.