High Performance Fluorescent Turn-On Probe for Amitriptyline Based on Hybrid Nanoassembly of Organic-Inorganic Nanoparticles.

Abstract

Fluorescence methods have gained enormous attention due to their ease in use, simplicity, selectivity and sensitivity. Fluorescent chemosensors respond instantly by converting molecular recognition to fluorescent signals. The consumption of pharmaceutical products by living beings is on great increase. The disposal of such compounds in the environment is a matter of great concern as these compounds enter aquatic environment and show accumulation in tissues of aquatic organisms. In the present study, we have utilized naphthalimide based receptors to fabricate organic nanoparticles (ONPs). These ONPs were used for the development of hybrid nanoassemblies, and the developed nanoassemblies were characterized with the help of transmission electron microscopy and dynamic light scattering studies. The photophysical studies were performed and the hybrid assembly developed using receptor 2 demonstrated a turn-on fluorescence emission behavior on binding with Amitriptyline. The present sensing system acted as promising candidate for determination of Amitriptyline among other contending drug molecules. The established system can recognize Amitriptyline up to a detection limit of 48 nM in aqueous medium. Electrochemical recognition studies show binding of hybrid nanoassembly of receptor 2 with Amitriptyline with limit of detection of 21 nM.