High performance, disposable hollow fiber flow field-flow fractionation for bacteria and cells. First application to deactivatedVibrio cholerae

Abstract

Interest in low-cost, analytical-scale, highly efficient, and sensitive separation methods for cells and bacteria has recently been increasing. Field-flow fractionation is well suited to the separation of different types of cells, including bacteria. High performance hollow fiber flow field-flow fractionation of such samples is demonstrated here for the first time with potentially disposable channels and high-sensitivity UV/Vis detectors. In this first application, hollow fiber flow field-flow fractionation is used to fractionate bacteria of biotechnological interest such as deactivated Vibrio cholerae, which are employed for whole-bacteria vaccine production. Quite short analysis times, high reproducibility, and low limits of detection are found. Retention of Vibrio cholerae is shown to depend on the mobile phase composition. Two serologically different Vibrio cholerae strains are partly distinguished by their fractogram profiles.