Abstract
Numerical calculations together with simplified analytical relations based on local equilibrium theory are used to determine the factors which govern the shape of the gradient formed during chromatofocusing when simple mixtures of buffering species are employed to produce linear or concave pH gradients. The numerical and analytical development is also used to determine the relation between the gradient shape and the buffering capacities of the adsorbed and liquid phases. Experiments which verify the theoretical methods are described where internally generated, retained pH gradients of various shapes are formed using high-performance chromatography columns. The resulting experimental and theoretical basis can be employed as means for the selection of the buffer composition for use in chromatofocusing.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have