Abstract
A high-performance capillary electrophoretic (HPCE) method with a UV−vis detector was used to determine kasugamycin content in commercial products. The results indicated that this method was capable of analyzing the kasugamycin content in formulated products with an instrument detection limit of 0.44 μg/mL and a method detection limit of 0.51 μg/mL. Recoveries of kasugamycin in formulated products were in the region of 99.9−103.2%. Relative standard deviation (RSD) values of HPCE determination of kasugamycin in formulated products ranged from 0.57 to 2.22%. All products collected from markets contained kasugamycin and were real products. High recovery, low detection limit, and low RSD values confirmed that the HPCE method is a sensitive and selective method. Thus the present official bioassay method could be replaced by the HPCE method. Keywords: HPCE; antibiotic fungicide; kasugamycin; detection limit; sensitivity; selectivity
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