Abstract
High-performance capillary electrophoresis (HPCE) was evaluated as a method for characterizing enzyme—antibody conjugates. Alkaline phosphatase (AP) and immunoglobulin G (IgG), individually and in a mixture, were used as standards to identify the components of an AP—IgG conjugate separation. 0.5% Methyl cellulose was added to the running buffer to separate and identify the conjugate, unreacted AP and unreacted IgG. Due to its speed and resolution, HPCE appears to be a good candidate for in-process evaluations of conjugates for immunoassays.
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