High-moisture corn grain silage inoculated with Propionibacterium acidipropionici and Lactobacillus plantarum in different storage times

Rafael Frank,Nathanael Cesar Costa Santos,Marcela Abbado Neres,Tatiane Fernandes,Guilherme Luis Silva Tesser,Clauber Polese,Cristine Kaufmann,Edinan Hagdon Cirilo,Ana Paula Guimarães Cruz Costa ,Nilton Rohloff ,Felipe Potenza Campos ,Rodrigo André Schöne ,Cleison De Souza ,T.l Köhler ,André Sanches De Ávila ,Ricardo Vianna Nunes

doi:10.33448/rsd-v10i1.10371

Rafael Frank, Nathanael Cesar Costa Santos + Show 14 more

Open Access

https://doi.org/10.33448/rsd-v10i1.10371

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Abstract

The aim of this study was to evaluate the chemical composition, growth of microorganisms, and the aerobic stability of high-moisture corn grain silage inoculated with Lactobacillus plantarum + Propionibacterium acidipropionici. The experimental design was completely randomized in a 2 x 5 factorial arrangement with four replications (50 experimental units) and the treatments were: use or not of microbial inoculant (2 x 105 colony forming unit (CFU) g-1 Lactobacillus plantarum + Propionibacterium acidipropionici) and the storage length for 0, 1, 3, 7, and 14 d. Aerobic stability of silage was evaluated at 28 and 56 d of storage length. The lactic acid bacteria population was influenced by storage length and the greatest values were estimated at 8 d of storage length. Regardless of inoculant application, no enterobacteria were present from 3 d post-ensiling. With respect to mold growth, an interaction between inoculant and storage length was observed wherein molds were most abundant after 3 d of storage in silage that received inoculant. However, at 14 d of storage the use of bacterial inoculant reduced the occurrence of molds. pH values obtained after 3 d of ensiling were less than 4.0 for all the treatments. The high-moisture corn grain silage possessed good fermentative quality. Further, adequate pH values were achieved from the third day of ensilage and were not influenced by the presence of bacterial inoculant.

Highlights

The use of high-moisture corn grain (HMC) silage has advantages compared to conventional grain which include harvest anticipation, the minimization of loss caused by transportation and reduced storage cost
Silage of high moisture corn grain is more susceptible to aerobic deterioration than grass and legume silages (Basso et al, 2012) and options are available for improving HMC fermentation as the use of microbial inoculants, which may facilitate silage conservation and enhance its quality (Saylor et al, 2020)
The aim of this study was to evaluate the chemical composition, development of principal microorganisms of HMC silage, and assess the aerobic stability of silage inoculated with L. plantarum plus P. acidipropionici

Summary

Introduction

The use of high-moisture corn grain (HMC) silage has advantages compared to conventional grain which include harvest anticipation, the minimization of loss caused by transportation and reduced storage cost. Silage of high moisture corn grain is more susceptible to aerobic deterioration than grass and legume silages (Basso et al, 2012) and options are available for improving HMC fermentation as the use of microbial inoculants, which may facilitate silage conservation and enhance its quality (Saylor et al, 2020). An alternative to improve the aerobic stability is the use of Propionibacterium sp., which can reduce silage deterioration, since this genus consume lactic acid and glucose, producing propionic and acetic acid as final fermentation products and both are compounds with antifungal action (Parizzi et al, 2012)

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