Abstract
BackgroundTo investigate the expression and significance of long noncoding RNA NORAD (lncRNA-NORAD) in breast cancer.MethodsQ-PCR was adopted to detect the mRNA expression of lncRNA-NORAD in breast cancer and adjacent tissues, survival analysis to compare the low-expression groups with the Kaplan–Meier method. Knockout of lncRNA-NORAD was adopted to observe the effects on the cell proliferation, migration and invasion of breast cancer in vitro and in vivo. The TGF-β/RUNX2 signaling pathway was observed by Western blot after the knockout of lncRNA-NORAD.ResultsIncreased expression of lncRNA-NORAD in breast cancer tissues promotes proliferation, invasion and migration of breast cancer cells and correlated with worse prognosis. LncRNA-NORAD activated TGF-β/RUNX2 signaling pathway in breast cancer cells.ConclusionsThese results strongly suggested that lncRNA-NORAD might play an important role in breast cancer progression and potentially be a new therapeutic target.
Highlights
To investigate the expression and significance of long noncoding RNA NORAD in breast cancer
Zhou et al Cancer Cell Int (2019) 19:63 proliferation, migration and invasion of breast cancer cells, and activated the transforming growth factor-β (TGF-β) signaling pathway. These results strongly suggested that Long non-coding RNA (lncRNA)-NORAD might play an important role in breast cancer progression and could be a therapeutic target
High lncRNA‐NORAD expression in breast cancer tissues correlated with poor prognosis Aberrant lncRNA-NORAD expression has been found in several cancers, we first detected lncRNA-NORAD expression levels in two breast cancer cell lines (MDAMB231and MCF-7) and the final results indicated that lncRNA-NORAD were higher than that in the normal mammary cell line (MCF10A) (Fig. 1a)
Summary
To investigate the expression and significance of long noncoding RNA NORAD (lncRNA-NORAD) in breast cancer. Breast cancer is the most malignant tumor in women through the world widely, and the incidence of breast cancer is still gradually rising, seriously threatening the women life and health [1]. Long non-coding RNA (lncRNA), a class of non-coding RNA molecules with about 200 nucleotides, plays an vital role in tumor growth, invasion, metastasis and angiogenesis [3]. Studies indicated that lncRNA-NORAD was valuable in the differential diagnosis of colorectal benign and malignant lesions [8]. High lncRNA-NORAD expression promoted EMT transformation and regulated transforming growth factor-β (TGF-β) signaling [9]. High lncRNA-NORAD expression in esophageal cancer was associated with poor prognosis [10]. The lncRNA-NORAD expression significance in breast cancer has not be investigated yet
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