High-level production and characterization of laccase from a newly isolated fungus Trametes sp. LS-10C

Abstract

Abstract An efficient laccase production fungus Trametes sp. LS-10C was isolated and identified, and the laccase producing medium was optimized by response surface methodology (RSM) in shake flask fermentation. The highest laccase activity obtained at 9 d in shake flask and 10 - L fermentator were 588.61±2.2 U mL −1 and 873.82±22.91 U mL −1 , respectively. The molecular weight of purified laccase determined by SDS-PAGE was around 65 kDa. The optimal pH was 4.0–4.5 and the optimal temperature was 40 °C. Among the tested substrates of ABTS, DMP and guaiacol, both the highest values of V max (11.4±1.5 mM min −1 ) and k cat / K m (6653.2±18.2 mM −1 s −1 ) were found for ABTS and the lowest K m value (38.1±1.7 µm) was found for DMP.