Abstract
A genetically modified XynA gene from Thermomyces lanuginosus was expressed in Pichia pastoris under the control of GAP promoter. P. pastoris expressed greater levels of xylanase (160IUml−1) on BMGY medium without zeocin after 56h. The xylanase production by recombinant P. pastoris was scaled up in a 5L fermenter containing 1% glycerol and the highest xylanase production of 139IUml−1 was observed after 72h. Further studies carried out in fermenter under controlled pH (5.5) yielded a maximum xylanase production of 177IUml−1 after 72h. The biobleaching efficacy of crude xylanase was also evaluated on bagasse pulp and a brightness of 47.4% was observed with 50IU of crude xylanase used per gram of pulp, which was 2.1 points higher in brightness than the untreated samples. Reducing sugars (24.8mgg−1) and UV absorbing lignin-derived compounds values were considerably higher with xylanase treated samples.
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