Abstract

Abstract Gene 51 and Gene 42 of Bacillus sphaericus 2297 were expressed in the nitrogen-fixing filamentous cyanobacterium Anabaena sp. 7120 on a shuttle vector. Intact recombinant cells showed high toxicity against larvae of Culex pipiens in laboratory and field tests, and lower toxicity against larvae of Anopheles sinensis . Although unselective culture could decrease the mosquitocidal activity, the genetically engineered Anabaena open up new possibilities for the use of cyanobacteria for mosquito control.

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