High interaction alginate–hyaluronate associations by hyaluronate deacetylation for the preparation of efficient biomaterials

Abstract

The paper presents fundamental investigations of alginate–hyaluronate association with significant polymer interactions for preparation of efficient biomaterials. For this purpose, acetamide functions of hyaluronate were partly cleaved by hydrazine at high temperature, yielding amino groups accessible to carboxylic functions of the alginate chain. Alginate–hyaluronate association was studied both in dissolved state by rheological measurements and CD, and in the form of gel slabs prepared after calcium diffusion. Appreciable interaction between carboxylic groups of alginate and the released amino groups of hyaluronate was put into evidence by enhanced values of the viscosity of mixed solutions, and by assessment of the properties of the gel formed: moderate deacetylation allowed gels of improved hardness and viscosity. Nevertheless, high deacetylation was observed to hinder the gel formation by Ca2+ complexation of alginate, by the significant competition of COOH–NH2 association. Interaction between alginate and modified hyaluronate results in regular gel structure, with small cavities. © 2000 John Wiley & Sons, Inc. Biopoly 54: 273–281, 2000