Abstract
We previously reported that the postgrowth of Escherichia coli K-12 after high-hydrostatic-pressure treatment (HPT) as moderate as 75 MPa for 30 min at 37 degrees C induced the formation of elongated cells due to an HPT-induced disorder in FtsZ ring formation, which is essential for cell division. Because an FtsZ ring is known as a bacterial cytoskeleton, we examined the effect of HPT on a eukaryotic cytoskeleton, such as actin cables (long bundles of actin filaments), of Saccharomyces cerevisiae. We found that actin cables disappeared after HPT (100 MPa) and were not reorganized until 3.5 h of growth after HPT. As long as actin cables disappeared, budding did not start. We also demonstrated that the in vitro polymerization of actin monomers was highly sensitive to HPT.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
