Abstract
Human bocavirus (HBoV) is a parvovirus and detected worldwide in lower respiratory tract infections (LRTIs), but its pathogenic role in respiratory illness is still debatable due to high incidence of co-infection with other respiratory viruses. To determine the prevalence of HBoV infection in patients with LRTI in Shanghai and its correlation with disease severity, we performed a 3-year prospective study of HBoV in healthy controls, outpatients and inpatients under five years of age with X-ray diagnosed LRTIs. Nasopharyngeal aspirates were tested by PCR for common respiratory viruses and by real time PCR for HBoV subtypes 1–4. Nasopharyngeal swabs from healthy controls and serum samples and stools from inpatients were also tested for HBoV1-4 by real time PCR. Viral loads were determined by quantitative real time PCR in all HBoV positive samples. HBoV1 was detected in 7.0% of inpatients, with annual rates of 5.1%, 8.0% and 4.8% in 2010, 2011 and 2012, respectively. Respiratory syncytial virus (RSV) subtype A was the most frequent co-infection detected; HBoV1 and RSVA appeared to co-circulate with similar seasonal variations. High HBoV viral loads (>106 copies/ml) were significantly more frequent in inpatients and outpatients than in healthy controls. There was a direct correlation of high viral load with increasing disease severity in patients co-infected with HBoV1 and at least one other respiratory virus. In summary, our data suggest that HBoV1 can cause LRTIs, but symptomatic HBoV infection is only observed in the context of high viral load.
Highlights
Human bocavirus (HBoV) was first discovered in the respiratory secretions of Swedish children with symptoms of acute respiratory infection (ARI) in 2005 [1]
HBoV DNA was detected by real time PCR in 39 out of 554 (7.0%) nasopharyngeal aspirates (NPAs) from inpatients, 3 out of 29 (10.3%) NPAs from outpatients and 5 out of 195 (2.6%) nasopharyngeal swabs from healthy controls
It is noteworthy that cough and abdominal pain were recorded in more inpatients with HBoV than inpatients without HBoV (89.7% vs 70.5%, p#0.05 and 7.7% vs 2.5%, p = 0.095 respectively; Table 1)
Summary
Human bocavirus (HBoV) was first discovered in the respiratory secretions of Swedish children with symptoms of acute respiratory infection (ARI) in 2005 [1]. HBoV has been associated with upper and lower respiratory tract infections (LRTIs) and gastroenteritis worldwide. Evidence is mounting that HBoV1 is an important cause of LRTIs with clinical symptoms including fever, cough, wheezing, dyspnea, abdominal pain and diarrhea [6,7,8]. Recent data show that HBoV1 is often shed for a long time after primary infection into the respiratory tracts of infants and young children. HBoV1 DNA and anti-HBoV1 antibodies in serum differentiate primary HBoV1 infection from long term post infection shedding [11], with detection of HBoV1 DNA in blood more closely associated with symptoms than positive respiratory samples alone [9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
