High-Gravity Fermentation for Bioethanol Production from Industrial Spent Black Cherry Brine Supplemented with Whey

Abstract

By-products from different industries could represent an available source of carbon and nitrogen which could be used for bioethanol production using conventional Saccharomyces cerevisiae yeast. Spent cherry brine and whey are acid food by-products which have a high organic matter content and toxic compounds, and their discharges represent significant environmental and economic challenges. In this study, different combinations of urea, yeast concentrations, and whey as a nutrient source were tested for bioethanol production scale-up using 96-well microplates as well as 7.5 L to 100 L bioreactors. For bioethanol production in vials, the addition of urea allowed increasing the bioethanol yield by about 10%. Bioethanol production in the 7.5 L and 100 L bioreactors was 73.2 g·L−1 and 103.5 g·L−1 with a sugar consumption of 81.5% and 94.8%, respectively, using spent cherry brine diluted into whey (200 g·L−1 of total sugars) supplemented with 0.5 g·L−1 urea and 0.5 g·L−1 yeast at 30 °C and a pH of 5.0 after 96 h of fermentation for both systems. The results allow these by-products to be considered low-economic-value alternatives for fuel- or food-grade bioethanol production.