Abstract
Abstract—Organogenic callus induction and high frequency shoot regeneration were achieved from cotyledon explants of teasle gourd. About 94.8% of cotyledon explants derived from 18 day old cotyledon produced green, compact nodular organogenic callus in MS medium containing BA 1 mgl-1 after 51 days with 95% shoot induction. The elongated shoots rooted in MS medium supplemented with IBA 0.3 mgl-1. Rooted plants were acclimatized in growth room and subsequently established in soil with 100% survivability. This protocol produced an average of 19.8 shoots cotyledon-1 explant in 22.2 days of culturing.
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