Abstract
Callus cultures of Agave sisalana were initiated from rhizome, and stem explants on MS, SH, Gamborg and White's medium supplemented with different concentrations of BA, kinetin, NAA, IAA and 2,4-D either in combination or singly. Optimum numbers of shoots were obtained from stem and rhizome explants either directly or from callus. The capacity for the shoot regeneration remained constant in the callus for more than 32 months. Regenerated shoots rooted readily within 21-35 days on fine sand with half the strength of inorganic salts of MS medium. 100% of the rooted plants successfully adapted to field conditions and grown in the soil. Regenerated plants were morphologically similar to the field grown mother plants.
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