High frequency of Human Polyomavirus 6 DNA in the cerebrospinal fluid of patients with neurological diseases

Abstract

No: 1743 Presentation at ESCV 2015: Poster 1 High frequency of Human Polyomavirus 6 DNA in the cerebrospinal fluid of patients with neurological diseases L. Signorini1,∗, F. Elia1, S. Villani1, R. Ticozzi1, M. Dolci1, D. Fraciotta2, E. Marchioni2, P. Ferrante1, S. Delbue1 1 University of Milano, Italy 2 Istituto Neurologico Mondino, Pavia, Italy Background: The family Polyomaviridae comprises thirteen human viruses that have been detected in various specimen types, mainly urine, skin and blood. Among all of them, only JC Polyomavirus (JCPyV), and sporadically BK polyomavirus (BKPyV) genomes have been detected in the Cerebrospinal Fluid (CSF). More recently, the unexpected finding of Human Polyomavirus 6 (HPyV6) DNA in the CSF from anHIV positive patientwith leukoencephalopathy was reported. JCPyV being a recognized cause of Progressive Multifocal Leukoencephalopathy (PML) in immunodeficient subjects provides a precedent for polyomavirus causing neurological symptoms, but alternative explanations should also be considered. The objectives of the study were to explore the prevalence of the newly discovered HPyV6, Human Polyomavirus 7 (HPyV7) andHuman Polyomavirus 9 (HPyV9) in neurological diseases, in comparison with those of JCV. Methods:CSFhavebeen collected from51HIV-positivepatients affected with HIV-related leukoencephalopathies and 191 HIVnegative patients affected with other neurological diseases (OND), such as meningitis, encephalitis, and encephalomyelitis. DNA was isolated and real-timePCRassays for JCV,HPyV6,HPyV7andHPyV9 were conducted. Results: JCV genome was detected in the CSF of 16/51 (31.4%) HIV-related leukoencephalopathies patients, HPyV6 genome in the CSF of 2/51 (3.9%) HIV-related leukoencephalopathies patients and in 37/191 (19.4%) HIV-negative patients with OND. HPyV7 and HPyV9 genomes were not amplified in any clinical specimens. Conclusions: HPyV6 genome was found in a high percentage of the CSF of the OND patients, whereas HPyV7 and HPyV9 genomeswere not detected. HPyV6 has not been previously associatedwith any disease, and it is nonetheless possible that theHPyV6 sequences originated from the skin during CSF collection. Whether HPyV6 detection in CSF is associated with patients’ neurological disease, is indicative of contamination with skin microbiota, or reflects increased blood-brain permeability is unknown. Demonstrating a causative role will require further studies. http://dx.doi.org/10.1016/j.jcv.2015.07.156 Abstract No: 1744 Presentation at ESCV 2015: Poster 1 Impact of rapid influenza diagnostic tests on the management of hospitalized patients with suspicion of flu L. Busson1,∗, M. Hallin2, M. De Foor1, B. Mahadeb1, O. Vandenberg3 1 Department of Microbiology, Iris-Lab, Brussels, Belgium 2 Department of Molecular Diagnosis, Iris-Lab, Brussels, Belgium 3 Infectious Diseases Epidemiological Unit, Public Health School, Universite Libre de Bruxelles, Brussels,