Abstract
Ras genes are thought to play an important role in human cancer since they have been found to be activated frequently in several types of tumors including breast cancer, where the overall incidence of K-RAS oncogene activation is 0-10%. Evaluation of K-RAS gene not only for mutational frequency but also for mutation types in this downstream signaling gene pathway is necessary to determine the mechanisms of action. The present study was conducted to test the hypothesis that K-RAS activation is involved in breast cancer risk of south Indian population. A total of 70 paired pathologically confirmed tumor and non-tumor tissues from the same breast cancer patients were analysed for most common K-RAS mutations of codon 12,13 and 61 by polymerase chain reaction followed by restriction digestion and direct nucleotide sequencing method. We found that a high rate of homozygous and heterozygous mutations of codon 12, but not codon 13 and 61, may influence the invasive ductal carcinoma of breast risk in this study. Our study indicated that only codon 12 may be involved in initiating breast carcinogenesis in India.
Highlights
The single most common proto-oncogenes disorder in human neoplasms is point mutation of RAS genes
Ras genes are thought to play an important role in human cancer since they have been found to be activated frequently in several types of tumors including breast cancer, where the overall incidence of K-RAS oncogene activation is 0-10%
The present study was conducted to test the hypothesis that K-RAS activation is involved in breast cancer risk of south Indian population
Summary
The single most common proto-oncogenes disorder in human neoplasms is point mutation of RAS genes. Previous investigators have reported that the mutational activation of ras oncogenes is likely involved in the etiology or progression of human breast cancer. These models were carried out by Chemical induction of breast adenocarcinomas in pubescent rats, it is a widely used model of carcinogenesis because the resulting tumors are histologically and behaviorally identical to human breast tumors. Knowledge of the functional implications of oncogenic mutations has increased our understanding of human carcinogenesis, through the commonalities as well as the differences between tumor types These results are addressed the mutational activation of K-RAS pathway in a single cohort of human tumor samples. In this study we assessed common mutations in codon 12, 13 and 61 by PCR followed by restriction Length Polymorphism and direct sequencing
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