Abstract
Objective To evaluate the stability and reliability of heart failure model by using high frequency Doppler echocardiography to early assess the stenosis of transverse aortic constriction (TAC) of mice. Methods Heart failure model on C57/BL6 mice was set up by TAC. By using high frequency Doppler echocardiography to detect the blood flow of ascending aorta and the site of constriction 3-5 days after TAC, together with ejection fraction and fraction shortening, molecular marker and histology result, the relationship between stenosis of constriction and heart function was analyzed. Results The survival rate of TAC mice after 8 weeks was 83.3%. The average stenosis of constriction was (75.8±1.3)%. Compared to Sham group, TAC mice got declined heart function after 2 weeks until 8 weeks. Histology results showed dilated heart and increased fibrosis. Atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), and β-myosin heavy chain (β-MHC) were markedly increased after 8 weeks. Conclusion High frequency Doppler echocardiography could early assess the stenosis of constriction in TAC mice and could contribute to evaluate the stability and reliability of heart failure model and to screen suitable samples for further research. Key words: High frequency Doppler echocardiography; Transverse aortic constriction; Heart failure
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