High Fidelity and Low Cost Detection of Multi-Color Fluorescence from Biological Cells in a Micro Integrated Flow Cytometer (MIFC) with Disposable Observation Cell

Y-C. Tung,S. J. Skerlos,K. Kurabayashi,C-T. Lin

doi:10.1007/978-94-010-0295-0_85

High Fidelity and Low Cost Detection of Multi-Color Fluorescence from Biological Cells in a Micro Integrated Flow Cytometer (MIFC) with Disposable Observation Cell

Y-C. Tung, S. J. Skerlos + Show 2 more

https://doi.org/10.1007/978-94-010-0295-0_85

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Publication Date: Jan 1, 2002

Affiliation: University of Michigan–Ann Arbor

#Disposable Microfluidic System #Total Analysis System + Show 8 more

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Abstract

Flow cytometry (FCM) has applications in biodefense, clinical, and environmental analysis of microbial populations [1]. Currently, the widespread use of FCM is limited by the size and cost of multi-color instruments. This paper describes a two-color micro-integrated flow cytometer (MIFC) which is novel with respect to 1) the configuration of fiberoptics within a disposable microfluidic system, and 2) the implementation of diode lasers, LEDs, and low-cost PIN photodetectors in a total analysis system with high signal to noise ratio (S/N). The MIFC (Fig. 1) has been validated using biological samples, and can reduce the size and cost of FCM by two orders of magnitude for portable, hand-held applications.

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