Abstract
BackgroundGlucose intolerance is a major health problem and is associated with increased risk of progression to type 2 diabetes mellitus and cardiovascular disease. However, whether glucose intolerance is related to impaired myocardial perfusion is not known. The purpose of the present study was to study the effect of diet-induced glucose intolerance on myocardial function and perfusion during baseline and pharmacological induced hyperaemia.MethodsMale Wistar rats were randomly exposed to a high fat diet (HFD) or control diet (CD) (n = 8 per group). After 4 weeks, rats underwent an oral glucose tolerance test. Subsequently, rats underwent (contrast) echocardiography to determine myocardial function and perfusion during baseline and dipyridamole-induced hyperaemia (20 mg/kg for 10 min).ResultsFour weeks of HFD feeding resulted in glucose intolerance compared to CD-feeding. Contractile function as represented by fractional shortening was not altered in HFD-fed rats compared to CD-fed rats under baseline conditions. However, dipyridamole increased fractional shortening in CD-fed rats, but not in HFD-fed rats. Basal myocardial perfusion, as measured by estimate of perfusion, was similar in CD- and HFD-fed rats, whereas dipyridamole increased estimate of perfusion in CD-fed rats, but not in HFD-fed rats. However, flow reserve was not different between CD- and HFD-fed rats.ConclusionsDiet-induced glucose intolerance is associated with impaired myocardial function during conditions of hyperaemia, but myocardial perfusion is maintained. These findings may result in new insights into the effect of glucose intolerance on myocardial function and perfusion during hyperaemia.
Highlights
Glucose intolerance is a major health problem and is associated with increased risk of progression to type 2 diabetes mellitus and cardiovascular disease
This study demonstrates that dietinduced glucose intolerance is associated with impaired myocardial function during conditions of hyperaemia, but not myocardial perfusion
Post-load blood glucose levels were increased in high fat diet (HFD)-fed rats compared to control diet (CD)-fed rats, whereas postload plasma insulin levels remained unchanged after HFD and CD feeding (Figure 3), indicating mild glucose intolerance
Summary
Glucose intolerance is a major health problem and is associated with increased risk of progression to type 2 diabetes mellitus and cardiovascular disease. The purpose of the present study was to study the effect of diet-induced glucose intolerance on myocardial function and perfusion during baseline and pharmacological induced hyperaemia. Contrast echocardiography is a non-invasive method for assessment and quantification of myocardial perfusion that is clinically applied for specific indications in the cardiology setting Using this technique it has been shown that myocardial blood flow reserve, as a measure for vascular function, is reduced in type 1 diabetic rats [8] and type 1 diabetic patients [9] when compared to normoglycaemic controls. In the present study we studied myocardial function and perfusion under baseline and pharmacological-induced hyperaemic conditions in a rat model for diet-induced glucose intolerance using contrast echocardiography. This study demonstrates that dietinduced glucose intolerance is associated with impaired myocardial function during conditions of hyperaemia, but not myocardial perfusion
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