Abstract
BackgroundThe purpose of this study is to investigate the effects of a high-fat diet from perilla oil on serum lipids, hepatic lipid metabolism and insulin sensitivity.MethodsMale Sprague–Dawley (SD) rats were fed either a control (CT) diet or a diet high in perilla oil (HP). After 16 weeks of feeding, the serum lipids were measured, and the gene expressions involved in hepatic fatty acid oxidation and synthesis were determined. In addition, hepatic fat deposition was detected, and insulin sensitivity was evaluated by means of euglycemic-hyperinsulinemic clamp.ResultsCompared with the rats in the CT group, the HP-feeding significantly decreased the levels of triglyceride (TG), total cholesterol (TCH) and HDL-cholesterol (HDL-c). HP-feeding did not change the levels of LDL-cholesterol (LDL-c), free fatty acid (FFA), intrahepatic lipids or body weight. Moreover, the HP-feeding dramatically increased the mRNA expressions of fatty acid oxidation markers (PPAR-alpha, CPT1A) and fatty acid synthesis markers (SREBP-1, FASN and ACC) in the liver. The HP-feeding induced increased protein levels of CPT1A, while reducing the protein levels of FASN and ACC in the liver. However, the glucose infusion rate significantly increased in the HP group compared with the CT group.ConclusionsOur data show that, in rats, excessive perilla oil intake may significantly lower serum lipids, strengthen hepatic fatty acid oxidation, and inhibit hepatic fatty acid synthesis, but at the same time may also lead to insulin resistance.
Highlights
Epidemiological evidence suggests that a western diet rich in fat is associated with obesity, NALFD and insulin resistance [1,2]
The purpose of the current study is to examine the serum lipids, hepatic fat deposition, insulin resistance, hepatic gene expression involving fatty acid oxidation and synthesis in high in perilla oil (HP)-induced rats
There was no difference in the body weights found between the HP and CT groups
Summary
Epidemiological evidence suggests that a western diet rich in fat is associated with obesity, NALFD and insulin resistance [1,2]. The characteristics of the animal models induced by high-fat diets from various fat sources are different. Two long-chain n-3 PUFAs (EPA and DHA) from fish oil have been widely recognized due to their beneficial effects on health, and are considered as essential supplements in human food [9]. Many studies have shown that n-3 PUFAs play roles in resisting inflammation, increasing insulin sensitivity, and reducing the incidences of obesity and cardiovascular and cerebrovascular diseases [10,11,12]. N-3 long-chain-PUFA has been shown to have a protective effect against insulin resistance [13]. The purpose of this study is to investigate the effects of a high-fat diet from perilla oil on serum lipids, hepatic lipid metabolism and insulin sensitivity
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
