Abstract
Expression of the plasmid pBR322-encoded tetracycline-resistant gene (tet) is known to cause other pleiotropic effects in addition to mediating the efflux of tetracycline from bacterial host cells. We have recently reported that expression of thetetgene in plasmid pKH47, a high-copy-number derivative of pBR322, causes growth inhibition ofEscherichia colicells harboring this plasmid. In this paper we report that reduced membrane-bound ATPase activity is found inE. colicells containing plasmid pKH47. This effect is dependent on the presence of an intacttetgene and reduces the ability of the cells to grow in a minimum medium containing succinate as the sole carbon source. The same effect is more dramatically observed in cells containing an unrelated plasmid in whichtetgene expression is under the control of thetacpromoter.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
