Abstract
Hepatocellular carcinoma (HCC) is a cancer with a poor prognosis and a low survival rate. Previous studies have found that microRNA-1266 (miR-1266) is associated with tumorigenesis and progression of several types of cancer, such as breast cancer and gastric cancer. The aim of the present study was to investigate the effects of miR-1266 on the clinical prognosis and biological behavior of HCC. For this purpose, reverse transcription-quantitative PCR was used to detect the expression of miR-1266 in HCC tissues and HCC cell lines. In addition, Kaplan-Meier survival analysis and Cox regression analysis were used to evaluate the prognostic value of miR-1266. Cell Counting Kit-8 (CCK-8) and Transwell assays were used to analyze the effect of miR-1266 on the biological behavior of cells. The aforementioned assays demonstrated that the examined HCC tissues had a significant upregulation of miR-1266 expression compared with normal tissues (P<0.001). The overexpression of miR-1266 was significantly associated with Tumor-Node-Metastasis stage (P=0.014). The results of the Kaplan-Meier analysis indicated that the 5-year overall survival rate of patients with high expression of miR-1266 was significantly lower compared with patients with low expression of miR-1266 (P=0.015). Cox regression analysis demonstrated that the expression level of miR-1266 could be used as an independent prognostic factor of HCC. CCK-8 and Transwell assays demonstrated that overexpression of miR-1266 promoted the proliferation, migration and invasion of HCC cells. In summary, the findings of the present study indicated that high expression of miR-1266 was positively associated with poor prognosis of patients with HCC and promoted cell proliferation, migration and invasion of HCC cells. miR-1266 may be used as a biomarker for HCC prognosis.
Highlights
Hepatocellular carcinoma (HCC) is one of the most common cancer worldwide, with similar rates of incidence and mortality [1,2]
The results indicated that the miR‐1266 expression level was significantly higher in the HCC tissues compared with the adjacent normal tissues (P
As a new type of biomarker, miRNAs serve an important role in the occurrence, development and metastasis of numerous types of cancer, such as HCC and prostate cancer [34,35]
Summary
Hepatocellular carcinoma (HCC) is one of the most common cancer worldwide, with similar rates of incidence and mortality [1,2]. As the third most common cause of cancer‐related death, HCC leads to >60,000 deaths globally each year [3,4]. The majority of patients with HCC are already in advanced stages when they are diagnosed, and the most effective treatment for early‐stage patients is surgical resection [5]. The long‐term survival rate of patients with HCC is low, and the prognosis is poor due to the high rate of HCC recurrence and metastasis so resection is not available for most patients [6]. Due to changes in lifestyle and environment, HCC is currently demonstrating a trend of continuously increasing global incidence that seriously affects quality of life [7]. It is important to identify effective targets for early detection, treatment, and prognostic monitoring of patients with HCC; it is necessary to identify new biomarkers [9]
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