Abstract
The overall incidence and mortality of renal cell carcinoma (RCC), the most common kidney cancer, are steadily increasing for reasons that are not fully explained. Our aim was to explore the expression of membrane MHC class I chain-related gene A (mMICA) in human RCC cell lines and tissue specimens, and to determine expression of soluble MICA (sMICA) in serum of patients with renal cell carcinoma, we used flow cytometry (FCM) and immunohistochemistry as well as an enzyme linked immunosorbent assay (ELISA) . The results showed that percentage of mMICA expression was significantly increased in human kidney cancer tissues and RCC cell lines (786-O and Ketr-3) than that in healthy adults and human embryonic kidney 293 (HEK293) cell line individuality (P<0.05). sMICA content in healthy adults was negative, but in renal cancer patients was significantly elevated (P<0.05). Our research showed that high expression of MICA in human kidney cancer, this results show that MICA might serve as potential tumor-associated antigen (TAA) in RCC.
Highlights
Renal cell carcinoma (RCC) is the most common renal cancer and represents approximately 2% -3% in all malignant tumor, a median survival of approximately 1 year (Cohen and McGovern, 2005; Sconocchia et al, 2009)
Our aim was to explore the expression of membrane MHC class I chain-related gene A in human RCC cell lines and tissue specimens, and to determine expression of soluble MICA in serum of patients with renal cell carcinoma, we used flow cytometry (FCM) and immunohistochemistry as well as an enzyme linked immunosorbent assay (ELISA)
The results showed that percentage of membrane MHC class I chain-related gene A (mMICA) expression was significantly increased in human kidney cancer tissues and RCC cell lines (786-O and Ketr-3) than that in healthy adults and human embryonic kidney 293 (HEK293) cell line individuality (P
Summary
Renal cell carcinoma (RCC) is the most common renal cancer and represents approximately 2% -3% in all malignant tumor, a median survival of approximately 1 year (Cohen and McGovern, 2005; Sconocchia et al, 2009). Annual incidence increased at about 2%, nearly 100, 000 cases of patients died of kidney cancer each year. About 85% of renal tumors are renal cell carcinoma and more than 85% of RCC are classified as clear cell carcinomas (CC-RCC). The overall incidence and mortality of RCC is steadily increasing for reasons not fully explained. Natural killer (NK) cells are a type of cytotoxic lymphocyte capable of mediating early innate immune responses to viral infections and recognition of transformed malignant cells (Diefenbach and Raulet, 2002). The cytolytic activity of NK cells against tumor cell lines was recognized very early and it is becoming evident that several characteristics of tumor cells may induce NK cellmediated antitumor reactivity (Herberman et al, 1975; Doubrovina et al, 2003)
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