Abstract
Objective: To investigate the changes of hsMAD2 protein and gene expression levels during chromosome segregation of human embryos. Method: The embryos of spontaneous abortion were collected in our hospital from 2009 to 2013, the chromosomal numbers of the embryonic villi were subsequently detected by fluorescence in situ hybridization (FISH). The patients were then divided into the normal and abnormal groups based on the chromosome number. The hsMAD2 protein and gene expression levels in the villi tissues of the two embryo groups were detected by western blotting and qRT-PCR, respectively. The hsMAD2 protein and gene levels in the embryonic villus tissue of the patient were detected. Results: From 2009 to 2013, we collected 50 embryos from spontaneous abortion patients. The chromosome abnormality and no abnormality were 36 cases (abnormal number of 28 cases (56.0%) and chimerism in 8 cases (16.0%)) and 14 cases (28.0%), respectively. The expression of hsmad2 protein and its gene in the villi of spontaneously aborted embryo with chromosomal abnormality in the abnormal group was significantly higher than that in those without chromosomal abnormalities (0.88 ± 0.20 vs 0.61 ± 0.19, P Conclusion: Abnormal number of chromosomes is closely related to spontaneous abortion Linked, hsMAD2 factor has a card effect on the cell cycle, can block the mitotic process of cells, and play an important role in maintaining the normal separation and stability of chromosomes.
Highlights
Spontaneous abortions account for 15% - 18% of clinically recognized pregnancies
The embryos of spontaneous abortion were collected in our hospital from 2009 to 2013, the chromosomal numbers of the embryonic villi were subsequently detected by fluorescence in situ hybridization (FISH)
Clinical research confirms its correlation to multiple factors, including heredity and immunity
Summary
Spontaneous abortions account for 15% - 18% of clinically recognized pregnancies. Its occurrence is mainly related to known factors such as heredity, infection, immunity, and environment, as well as other unknown factors [1] [2]. Human embryonic development is a gradual process. The early embryo lacks transcriptional activity; the embryonic gene is gradually formed and activated with the development of the embryo. The integrity of reprogramming, continuous cleavage, and formation and activation of the embryonic genome must be maintained for normal development of the human embryo [3] [4] [5]. Mitosis is a continuous process of nuclear restitution, and a basic form of realizing histogenesis and individual development. There is no correlation between hsmad and chromosomal abnormalities in spontaneous abortion embryos at home and abroad. Compare the expression of hsmad protein and gene between chromosomal abnormalities and normal fetal chorionic villi, in order to explore the effect of SAC on spontaneous abortion resulting from chromosomal abnormalities
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