Abstract
Enhanced degradation of cyclin-dependent kinase (CDK) inhibitor p27 Kip1 is known to be a powerful prognostic marker in many types of human cancers. Human CDK subunit 1 (Cks1) and S-phase kinase associated protein 2 (Skp2) are components of the SCF Skp2 complex, which acts as a ubiquitin ligase for p27 Kip1 . There are no reports about the involvement of Cks1 in the pathogenesis of human cancer. Here we show high expression of Cks1 in non-small cell lung cancers (NSCLCs) using Western blotting and quantitative real-time RT-PCR. The Skp2 mRNA expression level was high in squamous cell carcinomas and was inversely related with the p27 Kip1 protein level in individual clinical samples. In contrast, Cks1 mRNA expression had no such relationship with p27 Kip1 , although Cks1 mRNA was significantly elevated in adenocarcinomas. These results suggest that high expression of Skp2 and Cks1 may be involved in the pathogenesis of NSCLCs via different mechanisms.
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