Abstract
Background: Malignant fibrous histiocytoma (MFH) is a common type of soft tissue sarcoma and a serious threat to human health. MFH often relapses locally after the curettage is related to the residual cancer stem cells (CSCs). Currently, the dysregulation of microRNA (miRNA) has been found to be closely related to the recurrence of CSCs. However, whether dysregulations of miRNAs exist in MFH, CSCs remained unknown.Methods: In this study, miRNAs in MFH CSCs and MFH common cells were examined by gene probe. Then, target genes and their functions involved in the signal pathway were predicted by the relevant database. Finally, the miRNAs’ target regulatory network was constructed. Furthermore, the miRNAs and target genes were identified by quantitative polymerase chain reaction, whereas miRNA analogs and antagonists were transfected in tumor cells to investigate cell proliferation ability further.Results: Results showed that a total of 47 miRNAs were found, including 16 that were upregulated and 31 that were downregulated. The screened differential miRNA showed a different expression in the cell resistant strains compared with the control group. Quantitative polymerase chain reaction analysis confirmed that the relative abundance of seven miRNAs and four target genes varied significantly. The encouraging issue is that we found Hsa-miR-206 significantly inhibited MFH proliferative activity.Conclusion: Hsa-miR-206 played a key role in regulating MFH CSC properties that might be a representative marker and target for the diagnosis and treatment of MFH in the future.
Highlights
Malignant fibrous histiocytoma (MFH) is a common type of soft tissue sarcoma and a serious threat to human health
SPSS22.0 package analysis was used in this part, the selection of differential messenger RNAs (mRNAs) was analyzed by T-test with random variance model (RVM), and the difference was defined statistically significant if the Prediction of Target Genes and Their Function
Target gene function was obtained by the Gene Ontology (GO) database, which had 204 significant gene functions based on upregulated miRNAs and had 320 based on downregulated miRNAs
Summary
Malignant fibrous histiocytoma (MFH) is a common type of soft tissue sarcoma and a serious threat to human health. Malignant fibrous histiocytoma (MFH) is one of the most common malignant tumors in middle-aged and elderly patients, posing a serious threat to human health. MFH remains a locoregional relapse after traditional therapies, including chemotherapy, radiotherapy, or surgical removal of the tumor tissue. Previous studies have illustrated that these traditional therapies reduced the tumor but left some cancer stem cells (CSCs) behind, which are a unique subset of cells with the potential to self-renew, proliferate indefinitely, and differentiate into common tumor cells (Di et al, 2013; Tan and Zhang, 2016). The result showed that the ALDH+ subpopulation exhibits several characteristic CSC properties, including high clonogenicity and self-renewal, increased chemotherapeutic drug resistance, elevated expression of stemness and drug transporter genes, and high tumorigenic potential
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