Abstract
BackgroundRecent studies have revealed that serpin peptidase inhibitor clade E member 2 (SERPINE2) is associated with tumorigenesis. However, SERPINE2 expression and its role in lung adenocarcinomas are still unknown.MethodsThe expression levels of SERPINE2 in 74 consecutively resected lung adenocarcinomas were analyzed by using immunostaining. Inhibition of SERPINE2 expression by small interfering RNA (siRNA) was detected by quantitative PCR. Cell number assays and cell apoptosis assays were performed to clarify the cell-autonomous function of SERPINE2 in A549 and PC9 lung cancer cells.ResultsThe overall survival of patients with high SERPINE2 expression was significantly worse than that of patients with low SERPINE2 expression (P = 0.0172). Multivariate analysis revealed that SERPINE2 expression was an independent factor associated with poor prognosis (P = 0.03237). The interference of SERPINE2 decreased cell number and increased apoptosis in A549 and PC9 cellsConclusionThese results suggest that SERPINE2 can be used as a novel prognostic marker of lung adenocarcinoma.
Highlights
Recent studies have revealed that serpin peptidase inhibitor clade E member 2 (SERPINE2) is associated with tumorigenesis
SERPINE2 is overexpressed in a variety of adenocarcinomas, including breast cancer [3], pancreatic cancer [8], gastric cancer [9], and colorectal cancer [10], and its high expression is correlated with the degree of cancer malignancy
We Dokuni et al Respir Res (2020) 21:331 silenced SERPINE2 in two kinds of non-small cell lung cancer (NSCLC) cell lines using small interfering RNA (siRNA) and performed a cell number assay and evaluation of apoptosis to clarify the cell-autonomous function of SERPINE2 in lung adenocarcinoma cell lines
Summary
Recent studies have revealed that serpin peptidase inhibitor clade E member 2 (SERPINE2) is associated with tumorigenesis. SERPINE2 expression and its role in lung adenocarcinomas are still unknown. Serine proteinase inhibitor clade E member 2 (SERPINE2), known as protease nexin-1 (PN-1), was first identified as a neurite-promoting factor released by cultured glioma cells [1]. SERPINE2 is overexpressed in a variety of adenocarcinomas, including breast cancer [3], pancreatic cancer [8], gastric cancer [9], and colorectal cancer [10], and its high expression is correlated with the degree of cancer malignancy. In lung adenocarcinomas in particular, high expression of SERPINE2 has been previously reported [11], but the relationship to prognosis or disease progression has never been reported. We Dokuni et al Respir Res (2020) 21:331 silenced SERPINE2 in two kinds of non-small cell lung cancer (NSCLC) cell lines using siRNA and performed a cell number assay and evaluation of apoptosis to clarify the cell-autonomous function of SERPINE2 in lung adenocarcinoma cell lines
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