Abstract
The present investigation studied the protein quality of cookies developed for undernourished adolescents, following the storage stability of the cookies in different packaging materials and storage conditions. A total of 18 Sprague Dawley rats were divided into three groups (G1, G2, and G3) and each group was fed with a control diet, protein-free diet and cookies for 28 days, respectively. The Biological Value (BV), Net Protein Utilization (NPU), Protein Efficiency Ratio (PER) and True Protein Digestibility (TPD) were calculated. The developed cookies were packed and sealed in sterile Low-density polyethylene(LDPE) pouches and Metalized Polyester (M-PET) pouches. The packed cookies were stored at ambient conditions (24–27 ºC; 70–75% relative humidity (RH)) and accelerated conditions (37 ºC and 90% RH) for 90 days and analyzed for water activity, moisture content, color, texture, lipid oxidation and amino acid analysis. G3 groups provided with high-energy cookies recorded higher digestibility values and better PER, indicating that the product has better digestibility and good quality of protein and improves the growth of undernourished adolescents. Developed cookies packed in M-PET pouches under ambient conditions showed more effective quality retention with good texture, less lipid oxidation, better color and amino acid.
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