Abstract
The use of immobilized lipase from Candida antarctica (Novozymò 435) to catalyze ferulic acid of esterification was investigated in this study. The synthesis product was analyzed using HPLC. The results revealed that the major product was 2-ethylhexyl ferulate. Response surface methodology and 3-level-3-factor central composite rotatable design were adopted to evaluate the effects of synthesis variables, including reaction temperature (60℃- 80℃), enzyme amount (500 - 1500 PLU) and reaction time (8 - 24 h) on the percentage molar conversion of 2-ethylhexyl ferulate. The results showed that reaction temperature and reaction time were the most important parameters on percentage molar conversion. Based on ridge max analysis, the optimum conditions for synthesis were: reaction time 23 h, reaction temperature 71℃ and enzyme amount 1422 PLU. The molar conversion of actual experimental values was 98% under optimal conditions.
Highlights
Ferulic acid (4-hydroxy-3-methoxy cinnamic acid, FA) belongs to the family of phenolic acids and is very abundant in grains, fruits and vegetables
The reaction conditions were set at reaction temperature 70 ̊C, substrate molar ratio1 : 123, enzyme amount 1970 propyl laurate units (PLU), reaction time 24 h, 80 rpm, and 560 torr
The molar conversion increased when the reaction temperature and reaction time increased. These results revealed that reaction temperature and reaction time are most important parameters on the molar conversion of 2-ethylhexyl ferulate
Summary
Ferulic acid (4-hydroxy-3-methoxy cinnamic acid, FA) belongs to the family of phenolic acids and is very abundant in grains, fruits and vegetables. It has a capacity of antioxidation [1], excellent UV-absorbing, antitumor activity [2] [3], potential health benefits against cardiovascular problems, inflammatory diseases and cancer [4], which find a variety of applications in the pharmaceutical, cosmetic and food industries. A number of studies reported the enzymatic synthesis of hydrophobic derivatives of ferulic acid to increase its oil-solubility [9]-[11] Chemical synthesis of these derivatives is difficult, as FA is susceptible to oxidation under certain pH condi-
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