Abstract
We describe a concise and effective strategy towards precisely mapping Na(+)-K(+) ATPases on the cytoplasmic side of cell membranes by direct stochastic optical reconstruction microscopy (dSTORM). We found that most Na(+)-K(+) ATPases are localized in different sizes of clusters on human red blood cell (hRBC) membranes, revealed by Ripley's K-function analysis. Further evidence that cholesterol depletion causes the dispersion of Na(+)-K(+) ATPase clusters indicates that such clusters could be localized in cholesterol-enriched domains. Our results suggest that Na(+)-K(+) ATPases might aggregate within the lipid rafts to fulfill their functions.
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