High Efficiency Callus Induction and Regeneration of Solanum torvum Plants

Abstract

Callus induction and plant regeneration play a key role in transgenic technology. Although much progress has been made with respect to eggplant, this type of research is insufficiently developed in Solanum torvum (a wild relative of eggplant), which contains a large number of resistance genes. Here, a high-efficiency regeneration system of S. torvum was established. Stem segments and leaves were cultured on Murashige and Skoog (MS) medium supplemented with 0.5–3.0 mg·L−1 6-benzyladenine (6-BA) and 0.1–0.6 mg·L−1 α-naphthaleneacetic acid (NAA). The highest callus induction ratio (100%) was produced on MS + 1.0 mg·L−1 6-BA + 0.5 mg·L−1 NAA. The combination of 0.5 mg·L−1 BA and 1.0 mg·L−1 2,4-dichlorophenoxyacetic acid in MS medium (double microelement) was the best for plant regeneration. Well-developed shoots rooted on half-strength MS medium supplemented with 0.1 mg·L−1 indole-3-acetic acid (IAA). These results will be helpful for functional verification of resistance genes from S. torvum and may be useful to those working in the field of eggplant breeding.