High dose consumption of vitamin C for 8 weeks improved lipoprotein properties via enhancement of anti-oxidation and anti-glycation properties

Abstract

Although beneficial effect of high supplementation of vitamin C was well known, the physiological mechanism in serum is still under investigation. To investigate physiological effect of vitamin C in serum and lipoprotein on the viewpoint of anti-oxidant, anti-glycation, and anti-senescence activities. We analyzed blood parameters from young subjects (n=23, 21.7±2.0 years old) who consumed daily high dose vitamin C (1250 mg) for 8 weeks. Antioxidant activity of serum was enhanced with time dependent manner. In LDL fraction, apo-B48 band was disappeared at 8 week consumption in all subjects. In HDL, apoA-I expression was more enhanced by 20% at 8 week from initial level especially in male smoker. In lipoprotein fraction, all subjects showed remarkably diminished content of advanced glycated end products and reactive oxidized species (ROS). Uptake of LDL which was purified from each individual into macrophage was significantly reduced by 8-week consumption of vitamin C. Anti-senescence effect of HDL was remarkably enhanced after 8-week consumption. Expression pattern of microRNA 155 in HDL3 was 49% and 75% reduced in non-smoker and smoker, respectively. Melanin pigmentation facial skin was diminished after 8-week consumption, especially in smoker group. In conclusion, 8 weeks daily consumption of high dose vitamin C resulted enhancement of anti-senescence effect, skin whitening, and anti-atherosclerotic effect via improvement of lipoprotein parameters and microRNA expression with anti-oxidation and anti-glycation.