Abstract
To test the carcinostatic effects of ascorbic acid, we challenged the mice of seven experimental groups with 1.7 × 10-4 mol high dose concentration ascorbic acid after intraperitoneal administrating them with sarcoma S-180 cells. The survival rate was increased by 20% in the group that received high dose concentration ascorbic acid, compared to the control. The highest survival rate was observed in the group in which 1.7 × 10-4 mol ascorbic acid had been continuously injected before and after the induction of cancer cells, rather than just after the induction of cancer cells. The expression of three angiogenesis-related genes was inhibited by 0.3 times in bFGF, 7 times in VEGF and 4 times in MMP2 of the groups with higher survival rates. Biopsy Results, gene expression studies, and wound healing analysis in vivo and in vitro suggested that the carcinostatic effect induced by high dose concentration ascorbic acid occurred through inhibition of angiogenesis.
Highlights
Despite advances in medical science, both the number of cancer patients and the death rate due to cancer is increasing
Treatments with cancer cells and ascorbic acid Sarcoma 180 cells were cultivated in a CO2 incubator for five days, adding 9 ml RPMI 1640 medium, in 8 plates of 100 mm in diameter, and 5 × 105 cells in 200 ml phosphate buffer saline (PBS) were injected into the abdominal cavities of experimental mice using a 21 G injector
Group A was a control group that was treated with phosphate buffer saline (PBS), Group B was treated with low-level ascorbic acid at two-day intervals, and Group C was treated with high dose concentration ascorbic acid at two-day intervals
Summary
Despite advances in medical science, both the number of cancer patients and the death rate due to cancer is increasing. According to the work done by Levin's group [5,6], ascorbic acid has definite effect as an antitumor agent when administrated at a high dose concentration. Clinical case reports (from kidney cancer and bladder tumors) strongly indicate that high dose concentration ascorbic acid therapy in cancer treatment should be reassessed. These studies were confirmed by histopathologic review and examined in accordance with National Cancer Institute (NCI) Best Case Series guidelines [7]
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