Abstract
While high‐density DNA microarrays have been available for over three decades, the synthesis of equivalent RNA microarrays has proven intractable until now. Herein we describe the first in situ synthesis of mixed‐based, high‐density RNA microarrays using photolithography and light‐sensitive RNA phosphoramidites. With coupling efficiencies comparable to those of DNA monomers, RNA oligonucleotides at least 30 nucleotides long can now efficiently be prepared using modified phosphoramidite chemistry. A two‐step deprotection route unmasks the phosphodiester, the exocyclic amines and the 2′ hydroxyl. Hybridization and enzymatic assays validate the quality and the identity of the surface‐bound RNA. We show that high‐density is feasible by synthesizing a complex RNA permutation library with 262144 unique sequences. We also introduce DNA/RNA chimeric microarrays and explore their applications by mapping the sequence specificity of RNase HII.
Highlights
While high-density DNA microarrays have been available for over three decades, the synthesis of equivalent RNA microarrays has proven intractable until now
With coupling efficiencies comparable to those of DNA monomers, RNA oligonucleotides at least 30 nucleotides long can efficiently be prepared using modified phosphoramidite chemistry
We show that high-density is feasible by synthesizing a complex RNA permutation library with 262144 unique sequences
Summary
While high-density DNA microarrays have been available for over three decades, the synthesis of equivalent RNA microarrays has proven intractable until now. We describe the first in situ synthesis of mixed-based, high-density RNA microarrays using photolithography and light-sensitive RNA phosphoramidites. With coupling efficiencies comparable to those of DNA monomers, RNA oligonucleotides at least 30 nucleotides long can efficiently be prepared using modified phosphoramidite chemistry.
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