High-density lipoprotein subclass and particle size in coronary heart disease patients with or without diabetes.

Li Tian,Shiyin Long,Zhi Zeng,Mingde Fu,Chuanwei Li,Yinghui Liu,Yucheng Chen

doi:10.1186/1476-511x-11-54

Abstract

BackgroundA higher prevalence of coronary heart disease (CHD) in people with diabetes. We investigated the high-density lipoprotein (HDL) subclass profiles and alterations of particle size in CHD patients with diabetes or without diabetes.MethodsPlasma HDL subclasses were quantified in CHD by 1-dimensional gel electrophoresis coupled with immunodetection.ResultsAlthough the particle size of HDL tend to small, the mean levels of low density lipoprotein cholesterol(LDL-C) and total cholesterol (TC) have achieved normal or desirable for CHD patients with or without diabetes who administered statins therapy. Fasting plasma glucose (FPG), triglyceride (TG), TC, LDL-C concentrations, and HDL3 (HDL3b and 3a) contents along with Gensini Score were significantly higher; but those of HDL-C, HDL2b+preβ2, and HDL2a were significantly lower in CHD patients with diabetes versus CHD patients without diabetes; The preβ1-HDL contents did not differ significantly between these groups. Multivariate regression analysis revealed that Gensini Score was significantly and independently predicted by HDL2a, and HDL2b+preβ2.ConclusionsThe abnormality of HDL subpopulations distribution and particle size may contribute to CHD risk in diabetes patients. The HDL subclasses distribution may help in severity of coronary artery and risk stratification, especially in CHD patients with therapeutic LDL, TG and HDL levels.

Highlights

The diabetes is a major contributor to coronary artery disease (CAD) morbidity and mortality
Other study showed that small high-density lipoprotein (HDL) particle size was associated with an increased coronary heart disease (CHD) risk, but this association was largely explained by traditional risk factors [12]
The one-dimensional gel electrophoresis and immunodetection method Figure 1 shows the distributions of HDL subclasses for representative CHD and normolipidemic healthy subjects by nondenaturing 1-dimensional gel electrophoresis and immunodetection method

Summary

Introduction

The diabetes is a major contributor to coronary artery disease (CAD) morbidity and mortality. CHD patients often have increased small discoidal HDL particles and decreased large α-1 and α-2 HDL particles [10]. Other study showed that small HDL particle size was associated with an increased CHD risk, but this association was largely explained by traditional risk factors [12]. The progressive insulin resistance(IR) was associated a decrease in HDL size as a result of depletion of large HDL2a and HDL2b particles and a modest increase in small HDL. We examined the HDL subclasses distribution profile in CHD patients with or without diabetes by onedimensional gradient gel electrophoresis. We investigated the high-density lipoprotein (HDL) subclass profiles and alterations of particle size in CHD patients with diabetes or without diabetes

Methods

Results

Discussion

Conclusion