Abstract
Analysis of the expression of TNF-Rs on fresh peripheral blood mononuclear cells (PBMNC) by Scatchard analysis showed that Gr I (stages I and II) but not Gr II (stages III and IV) NHL patients have a significantly higher expression of surface TNF-Rs than normal controls. A rapid decrease in the binding of radiolabelled anti-p75 TNF-R Mab which gradually increased after 16h was seen in normal controls, while NHL patients showed a rapid increase in the binding of the Mab after activation of cells and a decrease in binding was observed only after 24h. Western blot analysis for normal controls showed a weak presence of the 42kDa fragment only, while the cytosolic extracts from fresh PBMNCs of NHL patients showed presence of both intact p75 TNF-R, as well as a 42kDa fragment corresponding to a soluble form of p75 TNF-R. Our results suggest that increased cytosolic pools of TNF-R in NHL patients might contribute to a rapid increase in its surface expression following activation of cells.
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