Abstract
There is an urgent need for rapid, accurate, and ultrasensitive diagnostics to identify respiratory viruses. Amplification-free detection of nucleic acids provides a promising technique for rapid diagnosis of viral infections. The objective of this study was to design, characterize, and optimize mutation-resistant molecular beacon probes for the detection of influenza versus SARS-CoV-2 viruses. High-coverage probe cocktails were computationally designed using our “fast evaluation of viral emerging risks” (FEVER) pipeline.
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