Abstract
A new fermentation system with continuous separation of inhibitory metabolites by cross-flow filtration was employed for high-concentration cultivation of homo-fermentative lactic acid bacteria in order to improve the productivity of cell-mass. The fermentation system allowed continuous removal of lactate, an inhibitory product, while retaining cells completely in the fermentor. By maintaining a low concentration of lactate in the medium, Streptococcus cremoris and Lactobacillus casei were cultivated to high concentrations of 81.5 and 49.0 g-dry cell weight per liter, respectively, giving cell productivities of 19-fold and 9-fold compared with the corresponding conventional batch cultivations. The time course of the cultivation with cross-flow filtration was predicted on the basis of the assumption that specific growth rate can be expressed as a function only of lactate concentration in the medium.
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