Abstract
C. utilis was continuously grown at cell densities of 76 to 80 g/l with yields of 0.46 to 0.55 g cell/g sugar, in a 10.5 m3 jet loop fermenter provided with a computer system for on line data acquisition and molasses flow rate feedback control. Infered ethanol production rate was employed as control variable to keep high conversion yields while maintaining high volumetric productivity (10.1 to 10.7 g/l h). Molasses addition control was useful to accommodate moderate gas holdup and OTR oscillations due to bubble coalescence inhibition and antifoam shot additions required for foam control. However, results suggest that improved fermentation process control will result, if gas holdup variations are minimized. A gas holdup control scheme is proposed including a differential pressure transmitter to indirectly measure gas holdup and provide an output signal to control antifoam addition. Advantages of such approach would be a more stable fermentation process continuously operating to maximum OTR and productivity.
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