Abstract
We describe a modification of the lactate biofuel cell to increase its capacity. A new multi-step enzyme reaction system without NAD was prepared using a bioanode modified with lactate oxidase, pyruvate decarboxylase, and pyrroloquinoline quinone-dependent aldehyde dehydrogenase. Its operating time was 6 times longer than that of the biofuel cell using the lactate oxidase-modified bioanode at constant current measurement of 1 mA cm−2 because of suppression of the diffusion overpotential due to the decrease in the fuel concentration.
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