High γ Amino Butyric Acid and Low Citrinin Produced by Monascus purpureus Serasi Strain

Abstract

The analysis bioactive production by Monascus purpureus SERASI strain was carried to know its potency for industrial application as herbal medicine. Previously, the fungus was cultured on malt extract agar for 12 days and harvested for inoculum purpose. For Monascus fermented rice (MFR) production, previously sterilized rice strain 24R after soaking treatment of plain water for a night was inoculated by the inoculum. Afterwards, the rice was incubated at 30°C for 6, 8, 10, and 12 days of incubation time period. The MFR was analysed for its GABA, lovastatin and citrinin content by using High Performance Liquid Chromatography. The result showed that the highest amino butyric acid (GABA) content of MFR was at 452 µg/g after 10 days of incubation time period. But it was decreased to 129 µg/g and 316 µg/g after 12 days and 14 days, respectively. While, lovastatin produce was at 315 µg/g after 10 days but decrease dramatically to 16 µg/g and 26 µg/g after 12 days and 14 days. In regard to citrinin, the acceptable content was reached at 0.12 µg/g after 12 days. At day 10 or 14 days, the citrinin content was higher at 13.39 µg/g and 14.18 respectively. Based on high GABA and low citrinin produces, this result showed that M. purpureus Serasi strain might be more potency for industrial purpose as an herbal medicine for anti hypertensive agent. Therefore, further study is needed to confirm MFR with high content of GABA as anti hypertensive agent.