Abstract
High-affinity membrane materials have been successfully synthesized through a combination of the polymer sulfonation reaction with transition metal counterion exchange treatment. This type of promising materials were embodied for the first time with the aid of dual-layer hollow fiber technology for protein separation. Three types of immobilized metal affinity membranes (Cu 2+, Ni 2+ and Zn 2+ forms) were developed in this work and they all exhibited enhanced protein separation performance compared to the as-spun hollow fiber in H + form due to the strong affinity between transition metal counterions and target protein molecules. Ultimately, the high-purity target protein (>99%, w/w) could be achieved via the membrane in Cu 2+ form.
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