HIF1 (rs11549465) and NFKB1 (rs28362491) variants association with obesity in Malaysia

Abstract

ObjectivesThe aim of this study was to do determine the role of variants in HIF1 (rs11549465) and NFKB1 (rs28362491) with obesity in Malay subjects. MethodsThis case-control study comprises of a total of 188 Malay subjects [93 obese (BMI > 30 kg/m2) and 95 normal (BMI18.5–22.9 kg/m2) as a control group]. Genotyping analysis of variants in HIF1 (rs11549465) and NFKB1 (rs28362491) was determined by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Serum samples were subjected to lipid profile (VLDL, HDL, CHOL, TRG, LDL and non-HDL) quantifications after determining the anthropometric indices (weight, height, waist circumference, hip circumference, Fat percentage and AS-index) using specific formulas. ResultsThe results were analysed for statistical rigors. Obese attributes had significantly greater HC (p < .001, M = 108.7), WC (p < .001, M = 94.94), TRG (p < .05, M = 1.04), CHOL (p < .05, M = 5.53), LDL (p < .001, M = 3.96), VLDL (p < .001), NON-HDL (p < .001, M = 4.33) as compared to normal, and obese group had lower serum HDL comparing with normal subjects (p < .001). Genotypic analysis of rs11549465 in HIF-1 was determined as genotypic frequencies (p < .001) of 84% (CC), and 16% (CT) in all samples. While for rs28362491 in NFKB1 reflected to be significant (p < .05); with Insertion (Ins) / Deletion (Del) (44.7%), Del/Del (30.3%) and Ins/Ins (25%) genotypes. There was significant association between NFKB1 (rs28362491) genotypes and obesity (p = .002); Del/Del genotype were less inclined to obesity, while Ins/Ins genotype attributes were found more inclined to be obese. ConclusionsThe synergistic effect of two genotypes (CC for rs11549465) and (Ins/Ins for rs28362491) were found to be a significant predictor for obesity beside WC as the anthropometric index and LDL in this study.