Abstract
The organization of the compartment of mesenchymal stem cells is still obscure. Two types of human stromal precursor cells are known. Both of them are analyzed in in vitro system: mesenchymal multipotent stromal cells (MMSC) and fibroblast colony forming units (CFU-F). The aim of this study was to compare the main characteristics of MMSC and CFU-F derived from the bone marrow of 24 healthy donors. Growth and differentiation parameters, as well as relative expression levels of different genes were analyzed in MMSC and CFU-F. MMSC were cultivated for 5 passages. CFU-F concentration was determined for each bone marrow sample. The data obtained demonstrated the heterogeneity and hierarchical organization of both studied populations of stromal precursor cells-MMSC and CFU-F. These two types of stromal precursor cells turned to be different in most parameters studied. Altogether MMSC seemed to be more immature cells than CFU-F and took up the higher position in hierarchical tree of mesenchymal stem cells. The rate of differentiation and proliferative potential decreased with the donor’s age in both populations MMSC and CFU-F.
Highlights
The aim of this study was to compare the main characteristics of mesenchymal multipotent stromal cells (MMSC) and CFU-F derived from the bone marrow of 24 healthy donors
Mesenchymal stem cells were identified by Arnold Caplan, who gave this name to the plastic-adherent stromal bone marrow cells and described their ability to differentiate into osteoblasts, adipocytes and chondroblasts [1]
Taking into consideration the gene expression data, we suggest that the whole MMSC population is represented by more imamture cells than CFU-F and can be positioned higher than CFU-F in mesenchymal stem cells hierarchy
Summary
Mesenchymal stem cells were identified by Arnold Caplan, who gave this name to the plastic-adherent stromal bone marrow cells and described their ability to differentiate into osteoblasts, adipocytes and chondroblasts [1]. The first direct evidence was that nonhematopoietic, mesenchymal precursor cells were present in the bone marrow originated from the work of Friedenstein and colleagues [2]. After 3 - 7 days, individual foci of two to four fibroblasts were observed among others These fibroblasts could differentiate into cells capable to form small deposits of bone or cartilage in vivo. These cells were termed fibroblast colony forming units or CFU-F. The frequency of CFU-F in bone marrow suspensions varied among species, and the results were influenced by the culture conditions (reviewed in [4])
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