Hidrojen peroksit ve mitokondrinin insan meme kanseri hücrelerine etkisi

Abstract

Purpose: It was aimed to evaluate the effect of hydrogen peroxide (H2O2), mitochondria isolated from human breast cancer cells (MCF-7) and human mesenchymal stem cells (IMSCs) on MCF-7 cell proliferation in this study.
 Materials and Methods: Mitochondria of IMSCs and MCF-7 cells were isolated according to the protocols specified in the kit and the protein amount of mitochondria was measured by the bicinchoninic acid method (BCA). H2O2 doses (0-1000 µM), mitochondria obtained from MCF-7 and IMSCs were transferred to MCF-7 cells and the viability was evaluated by tetrazolium salt (MTT) method. The cell percentages were calculated by measuring absorbance of the samples at 570 nm with a plate reader.
 Results: It was found that, ATP level of the mitochondria isolated from IMSCs was higher than MCF-7s. MCF-7 cell viability decreased significantly in 30 µM (%19), 40 µM (%26), 50 µM (%39), 75 µM (%39), 100 µM (%36), 200 µM (%28), 400 µM (%23), 800 µM (%22) and 1000 µM (%23) H2O2 doses. The transfer of IMSCs mitochondria caused a tendency to decrease in cell proliferation, but no significance was detected. But, it was found that healthy mitochondria transfer with 10 µM and 1000 µM H2O2 reduced the proliferation of MCF-7s (respectively %14 and % 8).
 Conclusion: It was determined that the transfer of healthy mitochondria isolated from IMSCs and H2O2 to MCF-7 is associated with proliferative processes, however transplantation of the healthy mitochondria into cancer cells is thought to be a promising new method in the treatment of the disease.