Hibrid biomaterials based on hydroxyapatite and blood components

Abstract

Hybrid biomaterials based on amorphous hydroxyapatite and blood components (fibrin, citrate plasma) were developed by chemical precipitation of hydroxyapatite in a biopolymer matrix (pH 11; Ca/P ratio 1.67) and by mixing 6–14 wt.% of hydroxyapatite gel (pH 7.0–7.2) with bipolymers. Chemically precipitated hydroxyapatite in biopolymer matrices is single phase or contains ticalcium phosphate impurity up to 30 %, mainly α-modification in fibrin matrix and β-modification in citrate plasma. The interaction of hydroxyapatite gel into the fibrin leads to significant amorphization of hydroxyapatite and an increase in its bioresorbability. Holding the composites with hydroxyapatite obtained by chemical precipitation in the Simulated Body Fluid model solution for 75 days leads to their partial resorption and simultaneous increase of biomimetic apatite, with its greater weight gain on composites with a fibrin. Hybrid biomaterials based on a fibrin obtained from the patient’s blood and hydroxyapatite gel showed positive result during implantation, allowing to form an adequate configuration of the defect, expanding the possibilities of ENT surgery.